Microbiome And Bioinformatics
Published 8/2022
MP4 | Video: h264, 1280x720 | Audio: AAC, 44.1 KHz
Language: English | Size: 429.01 MB | Duration: 0h 57m

Introduction to the Microbiome: Theory and Analysis
What you'll learn
Understand importance of the microbiome
Learn basic concepts in microbiome and bioinformatics
Analyze microbiome sequencing data
Use free, online, no-coding bioinformatics software
Requirements
High school level knowledge of biology and ability to use web browser.
No programming experience required.
Description
This course aims to build a foundation for understanding the background and methods involved in microbiome analysis projects.The course kicks off with an introductory practice test intended to gauge the student's background knowledge and set a baseline to estimate progress following course completion. It proceeds to introduce students to the value and relevance of this field of research. It will acquaint students with the basic underlying concepts of related disciplines such as molecular biology and sequencing.Students will also be introduced to the theory of sequencing techniques such as barcoding, multiplexing and demultiplexing. Various analyses methods and metrics commonly encountered in microbiome analysis will be discussed, such as taxonomic abundance and assignment, and diversity analysis. The theory segment wraps up with some helpful tips and suggestions on designing one's own microbiome experiment.After reviewing the theory, students will then have the opportunity to use a free online tool, EasyMap, to conduct their own microbiome analysis using demo data. The hands-on segment aims to solidify the concepts learnt over the theory lectures and also point students to an easy to use online resource to experiment and learn with.Finally, the course concludes with a comprehensive test that will evaluate the knowledge acquired by the students over the course.
Overview
Section 1: Introductory practice test
Section 2: Theory section
Lecture 1 Microbiome meaning and opportunities in the field
Lecture 2 Studying the microbiome
Lecture 3 Relevant background of molecular biology
Lecture 4 DNA sequencing and barcoding
Lecture 5 Abundance table and taxonomic assignment
Lecture 6 Diversity analysis
Lecture 7 Pointers to design a microbiome experiment
Section 3: Hands-on section
Lecture 8 Hands-on intro
Lecture 9 Hands-on part 1
Lecture 10 Hands-on part 2: Quality Control
Lecture 11 Hands-on part 3: Taxonomic analysis
Lecture 12 Hands-on part 4: Alpha Diversity
Lecture 13 Hands-on part 5: Beta Diversity
Lecture 14 Hands-on part 6: Taxa Differential Abundance and Function Prediction
Section 4: Final practice test
People interested to learn about microbiome and/or interested to analyze microbiome sequencing data using free online software without using any programming.,Beginners looking for a short course that will provide the necessary theory and hands-on tutorial.
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